Rapi:Direct™ Lysis Buffer
Makes 'Direct' PCR possible.
FEATURES
Fast and simple method for DNA extraction – whole process in 10 minutes
Minimized hands-on time makes it Ideal for on-site testing.
Various samples including whole blood can be used.
Rapi:Direct™ Lysis Buffer is a single tube DNA extraction system for rapid preparation of DNA from the variety of samples like cell, tissue, blood and serum which enables direct PCR. The formulation of Rapi:Direct™ Lysis Buffer works in two ways – maximizing the lysis efficiency of biological samples and suppressing inhibitory activities of crude lysates, which maintains the integrity of released genomic DNA. With its minimized hands-on time and good yields, Rapi:Direct™ Lysis Buffer is ideal for on-site PCR testing applications.
RESULTS OF PRESULTS OF PERFORMANCE VERIFICATION TESTS
PCR template volume
Lane M100bp DNA marker.
In this test, 10μl of whole blood sample was mixed with Rapi:Prep™ Lysis Buffer. After incubation under room temperature for 10 minutes, 1μl through 5μl of supernatant from the mixture were used as PCR template.
PCR template volume
Lane M100bp DNA marker.
In this test, 10mg of porcine tissue was mixed with Rapi:Prep™ Lysis Buffer. After incubation under room temperature for 10 minutes, 2μl through 5μl of supernatant from the mixture were used as PCR template.
PROTOCOL
ORDERING INFORMATION
You can download further information from below section.
